EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.39 | heat shock protein 90 | heat shock protein 90 enhances the affinity of wild-type enzyme to NADPH, L-arginine, and calmodulin but not to Ca2+ and BH4 | Bos taurus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.39 | overexpression of wild-type endothelial enzyme and mutant endothelial enzyme S1179D in Escherichia coli | Bos taurus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.39 | S1179D | mutation augments enzyme activity and increases its sensitivity to heat shock protein 90 (Hsp90). Mutation of Ser1179 to aspartic acid plays a dominant role in changing the sensitivity of the enzyme to NADPH. | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Bos taurus | endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.39 | Bos taurus | P29473 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.39 | aortic endothelial cell | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Bos taurus | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
1.14.13.39 | 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis | Bos taurus | 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.39 | endothelial nitric oxide synthase | - |
Bos taurus |
1.14.13.39 | eNOS | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 37 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.39 | 5.5 | - |
assay at | Bos taurus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.39 | physiological function | endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis | Bos taurus |