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Literature summary extracted from

  • Chen, Y.; Jiang, B.; Zhuang, Y.; Peng, H.; Chen, W.
    Differential effects of heat shock protein 90 and serine 1179 phosphorylation on endothelial nitric oxide synthase activity and on its cofactors (2017), PLoS ONE, 12, e0179978 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.39 heat shock protein 90 heat shock protein 90 enhances the affinity of wild-type enzyme to NADPH, L-arginine, and calmodulin but not to Ca2+ and BH4 Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.39 overexpression of wild-type endothelial enzyme and mutant endothelial enzyme S1179D in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.39 S1179D mutation augments enzyme activity and increases its sensitivity to heat shock protein 90 (Hsp90). Mutation of Ser1179 to aspartic acid plays a dominant role in changing the sensitivity of the enzyme to NADPH. Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Bos taurus endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.39 Bos taurus P29473
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.39 aortic endothelial cell
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
 Bos taurus 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?
1.14.13.39 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis Bos taurus 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.39 endothelial nitric oxide synthase
-
 Bos taurus
1.14.13.39 eNOS
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.39 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.39 5.5
-
 assay at Bos taurus

General Information

EC Number General Information Comment Organism
1.14.13.39 physiological function endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis Bos taurus